Hot-Start PCR 2XMaster Mix

                         Introduction of New Hot-start Technique of Taq DNA Polymerase

Our company developed a new proprietary hot-start technique, using chemically synthesized polymer, which can bind magnesium at less than 37℃ and release it immediately at higher temperature. No harsh condition to your template DNA, no possible animal source contamination, no worry for stability of reagents. Our product is provided as 2XPCR master format at present stage, easier for your PCR mulaaniption.

Our product supplies with red and yellow dyes, each reaction is for 50 µl PCR reaction volume, in most of situation, reaction volume could be reduced according to your experiments, and can be loaded to agarose directly for electrophoresis after PCR reaction. PCR enhancer and Taq DNA polymerase stabilizer are added in the mixture to increase amplification efficiency, it is suitable for amplification of difficult template, as our quality control, 4 kb fragment from genomic DNA and 6 kb fragment from lambda DNA can be amplified. The red mix can be freeze and thawed for 15 times without affecting PCR efficiency. But reducing times for freezing and thawing is strongly recommended. For frequent use, red mix can be stored at 4℃ for 2-3 months.

The size of dyes on different concentration of agarose:

Taq DNA polymerase is purified from recombinant E. coli source, no host genomic DNA contamination is detected by PCR amplification of E. coli single copy gene.