Taq DNA polymerase

Taq DNA Polymerase with Standard Taq Buffer

Cat. #	Size	Concentration	Price
T0001	2,000 unit	5,000 unit/ml	118 $
T0002	4,000 unit	5,000 unit/ml	209 $

• Isolated from a recombinant source
• Supplied with 10X Reaction Buffer
• Robust and reliable reactions
• Tolerates a wide range of templates
• Incorporates dUTP, dITP and fluorescently-labeled nucleotides
• Exceptional value in terms of cost per unit

Description:
Taq DNA Polymerase is a thermostable DNA polymerase that possesses a 5´→ 3´ polymerase activity and a 5´ flap endonuclease activity.

It is supplied with 10X Standard Taq Reaction Buffer。

Source:

An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1

Applications:

• PCR
• Primer Extension
• Microarray Analysis
• High-Throughput PCR

Reagents Supplied:

Standard Taq Reaction Buffer Pack (10X)

 

Enzyme properties:

 

Heat inactiviation:

 

No.

 

Reaction and Storage conditions:

 

Unit Definition:
One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C.

Unit Assay Conditions: 1X Reaction Buffer, 200 µM dNTPs including [³H]-dTTP and 200 µg/ml activated Calf Thymus DNA. 

Reaction Conditions: 
1X Standard Taq Reaction Buffer, DNA template, primers, 200 µM dNTPs and 1.25 units of Taq DNA Polymerase in a total reaction volume of 50 µl. 

1X Standard Taq Reaction Buffer:
10X PCR buffer:

100 mM Tris-HCl, pH 9.0, 500 mM KCl, 15 mM MgCl2, 1% Triton X-100

 

Concentration:
5,000 units/ml

Storage Conditions:
10 mM Tris-HCl
100 mM KCl
1 mM Dithiothreitol
0.1 mM EDTA
50% Glycerol
0.5% Tween-20
0.5% NP-40
pH 7.4 @ 25°C

Storage Temperature:
-20°C